Complete NMR characterization of lychnose from Stellaria media (L.) Vill

Lychnose (α-d-Gal-(1→6)-α-d-Glc-(1→2)-β-d-Fru-(1→1)-α-d-Gal) was isolated from Stellaria media, a representative member of the Caryophyllaceae plant family. Weak acid hydrolysis, enzymatic hydrolysis and complete NMR characterization were performed to confirm the identity of the tetrasaccharide. All 1H and 13C resonances were unambiguously assigned and the conformation of the sugars was determined using one and two dimensional NMR techniques. Anomeric characterizations in lychnose were confirmed from HMBC and NOESY spectra.