Enzymatic synthesis of dTDP-4-amino-4,6-dideoxy-d-glucose using GerB (dTDP-4-keto-6-deoxy-d-glucose aminotransferase) Original Research Article

Over-expressed GerB (dTDP-4-keto-6-deoxy-d-glucose aminotransferase) of Streptomyces sp. GERI-155 was used in the enzymatic synthesis of dTDP-4-amino-4,6-dideoxy-d-glucose (2) from dTDP-4-keto-6-deoxy-d-glucose (1). image Five enzymes including dTMP kinase (TMK), acetate kinase (ACK), dTDP-glucose synthase (TGS), dTDP-glucose 4,6-dehydratase (DH), and dTDP-4-keto-6-deoxy-d-glucose aminotransferase (GerB) were used to synthesize 2 on a large scale from glucose-1-phosphate and TMP. A conversion yield of up to 57% was obtained by HPLC peak integration given a reaction time of 270 min. After purification by two successive preparative HPLC systems, the final product was identified by HPLC and then analyzed by 1H, 13C, 1H–1H COSY NMR spectrometry.