Title of article :
Chemoenzymatic synthesis and application of a sialoglycopolymer with a chitosan backbone as a potent inhibitor of human influenza virus hemagglutination Original Research Article
Author/Authors :
Yutaka Makimura، نويسنده , , Shinya Watanabe، نويسنده , , Takashi Suzuki، نويسنده , , Yasuo Suzuki، نويسنده , , Hideharu Ishida، نويسنده , , Makoto Kiso، نويسنده , , Takane Katayama، نويسنده , , Hidehiko Kumagai، نويسنده , , Kenji Yamamoto، نويسنده ,
Issue Information :
دوهفته نامه با شماره پیاپی سال 2006
Abstract :
Sialoglycopeptide (SGP) is referred as the glycopeptide in hen’s egg yolk, which has an N-linked, complex-type, disialyl biantennary oligosaccharide with an α-(2→6)-sialyl N-acetyllactosamine residue. The residue is known as a binding ligand of type-A human influenza virus hemagglutinin. We describe herein a simple synthesis of a sialoglycopolymer with a chitosan backbone as a potent inhibitor of human influenza virus hemagglutination that makes use of the natural source ingredient, SGP, and the transglycosylation activity of endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M). Its inhibitiory activity for influenza virus hemagglutination is 40 times higher than that of SGP, and its competitive inhibition is determined to be over 300 times higher than that of fetuin. These results indicate that a sialoglycopolymer having a multivalent sialo-oligosaccharide could potentially be used for the prevention of influenza virus infection.
Keywords :
Chitosan , Hemagglutination , Sialo-oligosaccharides , Mucor hiemalis , Transglycosylation , Endo-M , Complex-type glycopeptide , Influenza virus
Journal title :
Carbohydrate Research
Journal title :
Carbohydrate Research