Structural and immunochemical studies of neutral exopolysaccharide produced by Lactobacillus johnsonii 142 Original Research Article

Abstract This paper describes the structure of neutral exopolysaccharide (EPS) produced by Lactobacillus johnsonii 142, strain of the lactic acid bacteria isolated from the intestine of mice with experimentally induced inflammatory bowel disease (IBD). Sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including two-dimensional 1H,1H COSY, TOCSY, NOESY, and 1H,13C HSQC experiments revealed that the repeating unit of the EPS is a pentasaccharide: →3)-α-d-Galp-(1→3)-β-d-Glcp-(1→5)-β-d-Galf-(1→3)-α-d-Galp-(1→3)-α-d-Galp-(1→ The rabbit antiserum raised against whole cells of L. johnsonii 142 reacted with homologous EPS, and cross-reacted with exopolysaccharide from Lactobacillus animalis/murinus 148 isolated also from mice with IBD, but not reacted with EPS of L. johnsonii 151 from healthy mice. Article Outline