Author/Authors :
Anna N. Kondakova، نويسنده , , Kseniya A. Novototskaya-Vlasova، نويسنده , , Marina S. Drutskaya، نويسنده , , Sof’ya N. Senchenkova، نويسنده , , Victoria A. Shcherbakova، نويسنده , , Alexander S. Shashkov، نويسنده , , David A. Gilichinsky، نويسنده , , Sergei A. Nedospasov، نويسنده , , Yuriy A. Knirel، نويسنده ,
Abstract :
Psychrotrophic bacteria of the genus Psychrobacter have not been studied in respect to lipopolysaccharide structure. In this work, we determined the structure of the O-specific polysaccharide of the lipopolysaccharide of Psychrobacter muricolla 2pST isolated from overcooled (−9 °C) water brines within permafrost. The polysaccharide was found to be acidic due to the presence of an amide of 2-acetamido-2-deoxy-l-guluronic acid with glycine (l-GulNAcA6Gly), which has not been hitherto found in nature. The following structure of the disaccharide repeating unit of the polysaccharide was established using composition analysis along with 1D and 2D 1H and 13C NMR spectroscopy:
Keywords :
Lipopolysaccharide , Bacterial polysaccharide structure , O-antigen , 2-Acetamido-2-deoxy-l-guluronic acid , Glycine , Psychrobacter muricolla
