Author/Authors :
Ahmadipour، Fereshteh نويسنده Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Vakili، Tahereh نويسنده Department of Clinical Biochemistry, Urmia University of Medical Sciences, Urmia, Iran , , Absalan، Abdolrahim نويسنده Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , , Mohiti-Ardakani، Javad نويسنده Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Hadinedoushan، Hosein نويسنده Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Khalili، Mohammadali نويسنده Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Pourrajab، Fatemeh نويسنده Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ,
Abstract :
Objective: GLUT4 is a type of glucose transporter and plays a central role in whole-body metabolism of carbohydrates. The muscle is the major site of GLUT4 and cell line models, to explore GLUT4 behaviors under new therapeutic approach, such as herbal components, should be evaluated. Here, C2C12 cell line is evaluated for GLUT4 translocation from intracellular compartment into the cell membrane.
Materials and Methods: C2C12 cell lines were cultured and differentiated into myotubes. Cinnamon/Turmeric-water soluble extract (CWE and TWE) were prepared and differentiated myotubes were exposed to the 100, 1000 ?g/ml CWE, 100 ?g/ml TWE or dimethylsulfoxide for 3 hours. Intracellular/cytoplasmic membrane compartments were separated using ultracentrifugation. GLUT4 percentages quantities were measured using western blotting. Data analysis of 3 sample sizes was done by comparing mean±SD of GLUT4 quantities and independent samples t-test.
Results: CWE enhances GLUT4 translocation from intracellular compartment into the cytoplasmic membrane; its effect is also dose-dependent meaning that 1000 ?g/ml concentration has a more potent effect than 100 ?g/ml (p < 0.05). However, 100 ?g/ml TWE had a reverse effect (p < 0.05).
Conclusion: Here we have shown that C2C12 is a good model for exploring GLUT4 changes under the effect of herbal extract. Induction or blockade of GLUT4 maybe under control of different signals transduction pathways. Furthermore, although turmeric ingredients are declared to have somewhat anti-diabetic effects, here we have shown that such effect is not applied via TWE effects on GLUT4 intracellular compartments movement into the cell membrane.
