Author/Authors :
Eleonora Fregolino، نويسنده , , Radka Ivanova، نويسنده , , Rosa Lanzetta، نويسنده , , Antonio Molinaro، نويسنده , , Michelangelo Parrilli، نويسنده , , Tsvetelina Paunova-Krasteva، نويسنده , , Stoyanka R. Stoitsova، نويسنده , , Cristina De Castro، نويسنده ,
Abstract :
Two cyclic forms of the Enterobacterial Common Antigen were isolated from Escherichia coli O157:H−. These antigenic determinants were purified from the biomass through extensive chemical, enzymatic and chromatographic procedures whereas MALDI MS spectrometry indicated their cyclic nature with a polymerization degree of 4 or 5. The two species, denoted as ECACYC-4 and ECACYC-5, were assigned by NMR and showed no further substitution with other appendages such as acetyl groups as usually described for similar cyclic antigens from other Enterobacteriaceae.
Keywords :
ECA , Enterobacterial common antigen , MALDI , NMR
