Author/Authors :
Yang Zou، نويسنده , , Mengyang Xue، نويسنده , , Wenjun Wang، نويسنده , , Li Cai، نويسنده , , Leilei Chen، نويسنده , , Jun Liu، نويسنده , , Peng George Wang، نويسنده , , Jie Shen، نويسنده , , Min Chen، نويسنده ,
Abstract :
A UTP-glucose-1-phosphate uridylyltransferase (SpGalU) and a galactokinase (SpGalK) were cloned from Streptococcus pneumoniae TIGR4 and were successfully used to synthesize UDP-galactose (UDP-Gal), UDP-glucose (UDP-Glc), and their derivatives in an efficient one-pot reaction system. The reaction conditions for the one-pot multi-enzyme synthesis were optimized and nine UDP-Glc/Gal derivatives were synthesized. Using this system, six unnatural UDP-Gal derivatives, including UDP-2-deoxy-Galactose and UDP-GalN3 which were not accepted by other approach, can be synthesized efficiently in a one pot fashion. More interestingly, this is the first time it has been reported that UDP-Glc can be synthesized in a simpler one-pot three-enzyme synthesis reaction system.
