Author/Authors :
Yu Zhou، نويسنده , , Yan-Song Li، نويسنده , , Bai-Hui Zhi، نويسنده , , Shi-Ying Lu، نويسنده , , Hong-Lin Ren، نويسنده , , Yuan-Yuan Zhang، نويسنده , , Zhaohui Li، نويسنده , , Qing-Feng Shen، نويسنده , , Xian-Mei Meng، نويسنده , , Zeng-Shan Liu، نويسنده , , Jun-Hui Zhang، نويسنده , , Ya-Ming Hao، نويسنده , , Wen-Di Liu، نويسنده , , Fang Song، نويسنده , , Dongming Yan، نويسنده ,
Abstract :
In order to detect nodularin (NOD) from water and fish samples, a monoclonal antibody (mAb) specific for NOD was selected which has no cross-reactivity with other toxins (<0.4%). Based on the mAb, a competitive enzyme-linked immunosorbent assay (ELISA) was developed. The working range of the ELISA was from 0.5 to 100 ng mL−1 with a detection limits of 0.16 ng mL−1. The average recoveries of NOD from sea water, flounder and blue mussels were 87.8%, 90.7% and 86.9%, respectively with the coefficients of variation (CVs) from 2.1% to 3.2%. Therefore, the developed competitive ELISA may become a new convenient and satisfied analytical tool for detecting of NOD in water and fish samples.
