Physicochemical and morphological characterization of different starches with variable amylose/amylopectin ratio

Starches have been analyzed in terms of their chemical composition, water retention capacity, morphological characteristics, and pasting/thermal properties. The changes in starch granule morphology during gelatinization were monitored by confocal laser scanning microscopy (CLSM). The different analysis revealed that waxy-starches (AP>90%) had a high water retention capacity (1.2–1.5 times higher) and developed higher paste viscosities (up to 40% for maize; 43% for barley). The swollen granules were highly susceptible to mechanical breakdown and solubilized faster. Higher AM contents showed inhibition of an extensive granule swelling and lowered the paste viscosity. The exceptional integrity of the high-AM starch even prevented its gelatinization at atmospheric pressure. Significant differences in physicochemical and morphological properties between the starches from regular, high-AM and waxy strains have become evident, no direct relationship between the AM/AP contents and the internal growth ring structures of the starch granules could be identified by CLSM. The waxy starches had a higher gelatinization temperature (up to 2 °C) and enthalpy (up to 20%), which indicates a higher crystalline and molecular order.