Serum nitrates and creatinine in workers exposed to atmospheric nitrogen oxides and ammonia

An epidemiological survey was carried out on 332 workers in a nitrogen fertilizer factory exposed to various levels of NO, NO2, NO3− and NH3. The population was divided into six groups as a function of the atmospheres in the different workshops and compared with a control group recruited outside the factory. We investigated the relations between the exposures of the different groups and serum levels of three nitrogen-containing derivatives involved in arginine metabolism: nitrates, creatinine and urea. The workers most exposed to the hydrogenated and oxygenated compounds of nitrogen were found to have the highest serum nitrates. Further analysis of the results showed that inhaled nitrogen oxides and ammonia were only partly responsible for the circulating nitrates. The excess nitrates were thought to have an endogenous origin due to inflammatory reactions induced by the pollutants, especially ammonia. In addition, we showed that the workers from the two groups exposed to both NO and NH3 had significantly elevated levels of serum creatinine (P<0.001). The excess creatinine was thought to have derived from the interaction of the exogenous NO with arginine metabolites.