In-Vitro Gene Disruption Using Tn7-Based Transposition: Evaluation of Its Utility and Efficiency in Generating a Brucella VirB1 Knockout

Vincent L. Wilson استاد مشاور , John R. Battista استاد راهنما , Philip H. Elzer استاد مشاور

PMN , Attenuation , goat , target immunity , virulence , in-vivo , Antibiotic resistance , Recombination , abortion , proteobacteria , Mutant , gene inactivation , neutrophil , transposon , virB1 , Brucella , mutagenize , Kanamycin , pBBR1MCS , facultative , Tn7 , cattle , cell free , abortus , mouse , phagocyte , melitensis , knockout , in-vitro , Vaccine , pGPS3 , Brucellosis , Ruminant , Mutagenesis , transposition , Gene disruption , virB , Macrophage , polymorphonuclear , Sheep , GPS-M , Tn5 , pGEMT