عنوان به زبان ديگر :
Expression of a Chimeric Protein Containing the Catalytic Domain of Shiga-Like Toxin and Human Granulocyte Mac rophage Colony-Stimulating Factor (hGM-CSF) in Escherichia coli and Its Recognition by Reciprocal Antibodies
پديد آورندگان :
Habibi Roudkenari Mehryar نويسنده , Bouzari Saeid نويسنده , Oloom Mana نويسنده , Jafari Anis نويسنده , Shahrokhi Nader` نويسنده , Shokrgoza Mohammad ali نويسنده
چكيده لاتين :
Fusion of two gen es at DNA level produces a single protein, known as a chimeric protein. Immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. Bacteri al or plant toxins are commonly used as the killing moiet ies of the chimeric immunotoxins. In this investigation, the catalytic domain of Shiga-like toxin (A1 ) was fused to human granulocyte macrophag ecolony stimulating factor (hGM-CSF) gene and the fused gene was then expressed using
an expression vector containing arabinose promoter. The protein thus obtaine d could be recognized by two different ELISA system designed for detection of hGM-CSF and Shiga-toxin and reconfirmed by
Western- blot. The recognition of the chimeric prote in by specific antibodies could be indicative of the proper form of the protein, which
justifies further steps to be taken to evaluate the potential effects of
the chimeric protein.
