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Genetic polymorphism of the melatonin receptor 1A locus in Iranian Shall and Karakul sheep

Ghiasi Heydar نويسنده , Nasiry Mohamad Reza نويسنده , Heravi Mousavi ALi Reza نويسنده , Mousavizadeh Abdol Azim نويسنده , Javad Manesh ALi نويسنده

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The genotypes of the melatonin receptor 1A(MTNR1A) were determined by PCR-RFLP in the native Iranian Shall and karakul breeds of sheep . Blood samples were collected from 60 karakul and 50 Shall breeds. Genomic DNAwas extracted based on the Guanidin Thiocyanate-slica gel method. After PCR reaction, PCR products were digested by the Mnl1 restriction enzyme. The MTNRIA locus had two genotypes with frequencies of 0.7 (+ +) and 0.3 (+ -) in the Karakul breed, 0.58 (+ +) and 0.42 (+ -) in the shall breed. Heterozygosis value for the MTNRIA locus in Shall and Karakul breeds were 0.42 and 0.3, respectively.This study provided evidence that sheep breeds, Shall and Karakul have variability in MTNRIA locus. Therefore, this locus can be used as a marker for the reproduction trait in selection programs.

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