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Fabrication of a Sulfite Biosensor by the Use of Coducting Polymer

Hosseini Mohammad نويسنده , Bahmani Babak نويسنده , Moztarzadeh Fatollah نويسنده , Rabiee Mohammad نويسنده

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In this research, an enzyme modified electrode has been produced during the electropolymerization ofaniline through incorporation ofSulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of the stability of the resulted sulfite biosensor revealed that formation ofa passive film on the aluminum surface causes improved stability ofthe electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35 יC, respectively. The apparent Michaelis-Menten constant and the activation energy ofthe enzyme catalyzed reaction were calculated.

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