مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Background: Various approaches have been used in the attempts to improve the quality of frozen.thawed mouse sperms. According to literatures, it seems that hyaluronic acid (HA) has an important role on the permeability and motility of sperms and their interaction with gametes. Objective: For evaluation of HA supplementation on sperm characteristics and fertilization capability, we investigated the effect of different doses of HA on mouse sperm morphology, motility, vitality and fertilization capability after freezing and thawing. Materials and Methods: The cauda epididymes was removed from 6 male mice with aseptic method. The sperm samples were frozen in 1.8 ml cryotubes with 18% raffmose and 3% skimmed milk containing cryo-protectant solution. HA at the concentration of 750 , 1000 or 1250 ug/ml was supplemented to frozen-thawed sperms. Sperm motility was measured with microscope, and fertilization rate was evaluated after routine IVF by counting the fertilized oocytes. For sperm morphology, papaniclau staining was used while; Eosin B was used for the assessment of sperm viability rate. Results: HA supplementation (750 ug/ml) improved motility parameters (p < 0.05) and increased the fertility rate (p < 0.05). The effect of 1,000 ug/rnl HA was also positive on the sperms. But 1,250 ug/rnl HA had negative effect on above mentioned characteristic. On the other hand, none of these doses had any effect on sperm morphology. Conclusion: The dose of 750 ug/ml of HA has the greatest effect on the motility, vitality and fertility rate of sperms after cryopreservation.