پديد آورندگان :
Jamshidzadeh Akram نويسنده , khoshnood mohammad javad نويسنده , dehghani zahra نويسنده , niknahad hossein نويسنده
چكيده لاتين :
The effects of different concentrations of the hydroalcoholic extract of dried powdered
leaves of Cichorium intybus L., on CCl4-induced hepatotoxicity in vivo in rats and CCI4induced
cytotoxicity in isolated rat hepatocytes were investigated. Rats received different
concentrations of the extract by i.p. injection for 3 consecutive days before the injection of
(3ml/kg) CCl4 (i.p.). Twenty four h after CCl4 injection the animals were sacrificed and the
livers were dissected for biochemical and histopathological studies. The results showed that
the Cichorium intybus extract could protect the liver from CCl4-induced damages with doses of
50 and 100 mg/kg, but concentrations higher than 200 mg/kg were less effective. For in vitro
studies, the extract were added to the suspension of freshly isolated rat hepatocytes incubated
in Krebs-Henseleit buffer under a gas flow of 95% O2 and 5% CO2, 20 minutes before the
addition of 10 mM of CCI4. The extract with concentrations of 60 to 600 ug Iml protected the
cells against CCkinduced cytotoxicity, but concentrations of 2: 1.5 mg/m1 and higher increased
the CC14-induced cytotoxicity. The Cichorium intybus extract itself was toxic towards isolated
hepatocytes in concentrations above 3.6 mg/ml. The results of the present study therefore
supported the traditional believes on hepatoprotective effect of the Cichorium intybus extract,
however, high concentrations were hepatotoxic.
