مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

The effect of Odonthobuthos doriae (O.d) scorpion venom at 0/3, 1 and 3, 10 ug/ml concentrations were investigated on nerve-muscle transmi ssion, using the Twitch tension technique.A concentration of 0.3 ug/ml caused a small change in the twitch height in response to indirect muscle stimulation, but higher concentrations (1, 3, 10 ug/rnl) caused a transient augmentation in twitch response followed by a large contracture in the chick biventer cervices (CBq preparation. This effect could be defined as a complex action ofthe venom, predominately presynaptic, in which itsי effects on postjunctional synapses is also maintained. In order to find out which bioactive fraction could explain the venom effects , the soluble crude venom was partially separated by the gel filtration method, using a Sephadex Gso column, and four fractions were separated. Two of the four purified fractions (O.d F3, O.d F4) were characterized as toxic and their LDsovalues were lower than the crude venom. Unlike the O.d FI and O.d F2 fractions, O.d F3 and O.d F4 fractions caused a significant block in the twitch and contracture, in comparison to the control sample . in conclusion, fractions O.d F3 and O.d F4 are supposed to be as the biological activ e components of the O.d . venom.