چكيده لاتين :
Giardia lamblia is one of the most common intestinal protozoan parasites infecting human in the world. The goal of this study was searching for in-vitro effect of folic acid and cobalamin on adhesion and growth of G.
lamblia as two important mechanisms in the pathogenesis in TYI-S-.33 medium. G. Lamblia trophozoites were obtained by in- vitro excystation procedure. Three groups of Giardia trophozoites were analyzed: control
group, G.lamblia was cultured in TYI-S-33 without any vitamin, 2nd group with 0.1 mug/ml vitamin B12 or folic acid, and yd group with 0.5 mug/ml of vitamin B12 or folic acid. All culture media tubes incubated at
.3 7 degree C. After 2 h of incubation. the adherence into borosilicate culture tubes, and after 24 h the growth oftrophozoites were measured .The results showed that in vitamin B12 groups, the growth was increased significantly (P< 0.05) but the adherence decreased significantly (P< 0.05). Folic acid inhibited the growth rate significantly (P< 0.05), but it increased adherence in axenic culture significantly (P<0.05). The results showed that vitamin B12 and folic acid altogether might reduce pathogenesis of G. lamblia by reducing adherence and growth, respectively.
