چكيده لاتين :
The effects of pesticide endosulfan (an organochlorine compound), on the ovaries of
bluegill fish (Lepomis macrochirusi were studied.. Exposures for 24 hs with histological preparations
at 25% (0.25 JlgIL), 75 % (0.75 JlgIL), and 100 % (1 JlgIL) sub lethal concentrations were examined.
The control contained an abundance of the different stages of oocytes(OocytesI, II, III, and IV) and
had an intact ovigerous lamellaeand follicularlining. The control also contained a thick and complete
ovarian wall with evident provitelline and euvitelline nucleoli. After 24 hr exposure to a 25%
concentration, many Oocyte II and III cells had damaged stroma and cytoplasmic and nuclear
retraction. Adhesion is pronounced at the 25% concentration, but is even more profound at the 75%
concentration. Empty fol1icles and a unique cytoplasmic clumping can be observed in Oocyte III
and IV cells in the 75% concentration. The ovaries offish exposed to a 100% concentration display
an immense amount of empty follicles along with necrosis of nuclei and expel1ed nuclei. As the
concentration increased, the amount of atretic cells increased, and the ovarian wall became more
thinned and lifted. Macrophages were more evident as the concentration increased and the sizes of
the different stages ofoocytes,exposed to the different concentrations became smaller as well. This
study showed that there is a clear correlation between the amount of damage seen and the amount
of endosulfan .
