چكيده لاتين :
In this study, the effects of three structural analogues of adenosine upon proliferation of human
tumor cells were investig ated. Pre vious research showed a cytotoxic effect of adenosine via A3 receptor and
At receptor and sometimes this effect was receptor independent. The researches showed a differential
cytotoxic effect of adenos ine and its A3 agonists on cancerous cells, while other studies demonstrated tumor
promoting effect of adenosine and its AI agonists. The purpose of the present study was the evaluation of the
possible selective anti-tumor effect of AI receptor agonists on cancerous cell s. Methods: The substances of
Ns-cyclohexyl-adenosine (CHA, AI agonist), R-isomer of Ns-phenylisopropyladenosine (R-PIA, AI agonist)
and N5-ethylcarboxamido-adenosine (NECA, adenosine AI-A 2non-specific agonist) were tested for their antiproliferative
effect using 3-(4,5-dimethyIthiazol-2-yl)-2,5-diphenyltetrazol ium bromide (MTT) assay method.
Hep G2, Hep2, CAC02, ACHN and L929 cell Jines were used in this assay . Results: CHA inhibited cell
proliferation in three cell line s (in concentration of 5-50 uM) and R-isomer of R-PIA in one cell line
(in concentration of 10-50 uM) . These effects were inhibited parti ally by addition of 1,3-Dipropyl 8-
cyclopentylxanthine (A I antagonist). The NECA analogue had no inhibitory effect on the ce ll
proliferations. All of the substances had no cytotoxic effect on L929 ce lls (mouse connecti ve tissue fibroblast
cell line ). Conclusion: CHA and R-PIA had inhibitory effect on the proliferation of human tumor cell line s
partially via AI receptor, while they didnיt show such effect on fibrobla st cell s. These results suggest that AI
adenosine receptor agoni sts have a good potential of speci fie anti-tumor activity. fran. Biomed. 1. 12 (4) : 203208,
2008
