چكيده لاتين :
Gadolinium-porphyrins have been synthesized and are currently being investigated as magnetic resonance
imaging (MRI) contrast agents . This study aimed to synthesize Gd-hematoporphyrin and applicate it for in vitro
detection of breast cancer cell line (MCF-7).
Methods: The naturally occurring porphyrin (hematoporphyrin) was inserted with gadolinium (III) nitrate hexahydrate
to yield Gd-H. T1 relaxation times and signal enhancement of the contrast agents were presented, and the results were
compared. UV spectrophotometer measured the attachment of Gd to the cell membrane ofMCF-7.
Results: Most of gadolinium chloride (GdC13) was found in the washing solution, indicate that it didn יt fixed to the
breast cell membranes during incubation. Gd-DTPA showed some uptake into the MCF-7 cell membranes with incubation,
however, its uptak e was significantly lower than Gd-H.
Conclusion: Good cell memberan uptake of Gd-porphyrin is comparable to controls, indicating selective delivery it to
the breast cell line and considerable potency in diagnostic MR imaging for detection of breast cancer.
