عنوان به زبان ديگر :
In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility
چكيده لاتين :
Background: Nicotine, cotinine and cadmium are harmful components of cigarettes that have an
effect on human reproductive function. Although the effects of cigarette smoke on male reproductive
function is characterized in several articles its mechanism ofaction is stilIunknown.
In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm
creatine kinase activity in vitro.
Materials and Methods: Total creatine kinase activity is measured in sperm homogenates after
chromatography on a diethylaminoethyl cellulose (DEAE-32) column.
Results: Weshow that creatine kinase activity is signifi cantly inhibited by nicotine (44%), cotinine
(39%) and cadmium (65%) at a concentration of 60 ug/rnl. Kinetic studies reveal that the inhibitory
effect of nicotine, cotinine and cadmium are competiti ve in relation to creatine phosphate .
Conclusion: Considering the importance of creatine kinase activity for normal sperm energy
metabolism, our results sugge st that inhibition ofthis enzyme by nicotine, cotinine and cadmium may
be an important mechanism in infertility amongst male smokers . However, further investigations
are needed to elucidate the exact mechanism of cigarette effect on male reproductive function at
the molecular level.
