چكيده لاتين :
High performance liquid chromatography coupled with photo diode array detector (HPLCDAD)
has been reported to quantify isolated flavonoids or these compounds in complex
biological matrices, such as Chinese herbal drugs and products from factories. This work was
designed, therefore, to develop an HPLC-DAD system to separate quercetin, luteolin and
apigenin and to quantify them in extractive solutions from Marchantia convoluta. Flavonoids
were analyzed on a Kromasil RP-C I8 column; using a mobile phase, consisted of methanolacetonitrile-
acetic acid-phosphoric acid-H 20 (200: 100:10:10:200, V/V); under the following
conditions: detecting wavelength, 352 nm; flow rate, 0.60 ml/min; the sensitivity, 0.05 AUFS
and the volume of injecting sample, 6.0 Ill. The HPLC system was operated at ambient
temperature (28±1 0C). The method showed linearity for quercetin, luteolin and apigenin in the
range 2.0-20.8, 2.2-24.0 and 1.6-20.0 ug/ml respectively, and the R.S.D. of the slope of the
three lines was, respectively, 0.33%, 1.21% and 2.49% for quercetin, luteolin and apigenin.
The aqueous and ethanol 80% extractive solutions showed linear response 1.5-15 ul/ml and
ethanol 50% extractive solution in range 1.0-10 ul/ml, Precision and accuracy were determined
for ethanol 80% extractive solution, in concentration of 10 ul/ml. The recoveries were 95.9298.10%,
92.18-95.13% and 98.72-103.19% for quercetin, luteolin and apigenin respectively.
RSD of results was 2.83-3.62%. The HPLC method showed an excellent performance in
separating the flavonoids quercetin, luteolin and apigenin in Marchantia convoluta extracts,
since the presence of interference has been previously evaluated and the mobile phase was
chose carefully
