19736

An Improved GC Method for Rapid Analysis of Valproic Acid in Human Plasma Without Derivatization

Ahmadkhaniha Reza نويسنده , Rastkari Noushin نويسنده , Kobarfard Farzad نويسنده , Pakdaman Hossein نويسنده , Ahmadkhaniha Omid نويسنده , Kebriaeezadeh Abbas نويسنده

37

42

6

VPAwas extracted into chloroform with suitable recovery. By using Stabilwax®-DA capillary GC column, a symmetrical gas chromatographic peak was obtained without the need for derivatization. The calibration curve was proved to be linear (r2 = 0.998) in a wide concentration range (0.45-100 ىg/ml). Inter-day and intra-day accuracy and precision of this method was investigated during the method validation and the method has good precision and accuracy. This method is highly reproducible with a limit of detection 150 ng/ml of VPAin human plasma and could be used in TDM and pharmacokinetic studies.

1203749