چكيده لاتين :
Atorvastatin has been shown to be cardioprotective in ischemia-reperfusion (I/R) injury. Inhibition of
Fas expression prevents I/R induced apoptosis. However, the influence of atorvastatin on Fas expression in I/R injury
was not studied. Therefore, we designed this study to see the influence of atorvastatin on cardiomyocyte apoptosis and
Fas expression following acute I/R in vivo.
METHODS: Thirty Wistar rats were selected and divided into three groups (n = 10): acute ischemia-reperfusion (I/R)
group, acute ischemia-reperfusion and treated with atorvastatin group and sham-operated group. Apoptosis of the cardiomyocytes
was observed under electron microscopy and determined by optic microscopy with TUNEL (terminal deoxynucleotidyl
transferase (TdT)-mediated dUTP nick end-labeling) staining. To detect the expression of Fas in the
cardiomyocytes, immunohistochemistry method was used. Image analysis system was used to quantitatively estimate
the positive metric substances of immunohistochemistry through the mean optic density.
RESULTS: Numerous apoptotic cardiomyocytes were found in ischemic fields in ischemia-reperfusion groups and
weren’t observed in the sham-operated group. Fas expression was significantly higher in the ischemia-reperfusion group
as compared to sham-operated group, but was decreased significantly in atorvastatin treated group as compared with I/R
group.
CONCLUSION: Upregulation of Fas expression in myocardial ischemia-reperfusion can induce cardiomyocyte apoptosis,
and atorvastatin can significantly inhibit cardiomyocyte apoptosis by inhibiting Fas expression.
