چكيده لاتين :
There has been much interest generated in the recovery
of nanoparticulate (nanoparticle) bioproducts
(Second generation of biotechnological products) such
as plasmid DNA and viruses as putative gene therapy
vectors, macromolecular assemblies as drug delivery
vehicles and virus-like particles as vaccine components.
Such product must be manufactured in
advanced stages of purity, material definition and
sophisticated formulation to rival those demanded of
the pharmaceutical macromolecules which dominate
as the first generation products. Nanoparticulates are
characterized by a critical size range (10-300 nm
diameter) and complexity of surface chemistry and
internal organisation which pose new challenges in
separation science and engineering, controlled
chemistries of modification and material measurement
not readily addressed by extant technologies. Current
review article is concerns with structural characterisations
of nanoparticulate bioproducts as well as redesign
of their downstream processing techniques
which are common to all programmes. This focus is
upon candidate partition systems which can contribute
to the fractionation, recovery and purification of
nanoparticulate assemblies from their soluble components
(capsid proteins from virus, polynucleotides from
plasmid DNA, soluble, agglomerated forms of protein
etc.). The mechanistic design of new separation and
formulation technologies based upon a sound understanding
of quantifiable structural features of these
nanoparticle bioproducts is strongly indicated.
