عنوان به زبان ديگر :
Expression of regulated oncogen-alpha by primary hepatocytes following isolation and heat shock stimulation
پديد آورندگان :
Gholamhossein Hassanshahi نويسنده , Rezvani Mohammad Ebrahim نويسنده , Kazemi Arababadi Mohammad نويسنده , Shamsizadeh Ali نويسنده , Mahmoodi Mehdi نويسنده , Mousavi Ali نويسنده , Dickson Alan James نويسنده
چكيده لاتين :
High levels of regulated oncogen-alpha (GRO-ƒ؟) expression
have been observed in the liver. GRO-ƒ؟ stimulates proliferation
of epithelial cells and induction of rolling and extravascular
migration of neutrophils and mononuclear cells. Given the
above observations, this chemokine was chosen to be analyzed
in freshly isolated and cultured hepatocytes. In this
study, hepatocytes (2پ~106 cell/ml) were isolated from male
Sprague Dawley rat liver and cultured on plates that were
pre-coated with collagen type-I matrix. The western and
northern blot analyses were employed to detect GRO-ƒ؟ at
the protein and mRNA levels in freshly isolated and cultured
hepatocytes in response to isolation and heat shock stresses.
GRO-ƒ؟ was shown to be expressed by isolated rat hepatocytes
immediately after isolation and early culture and
decreased with time. mRNA was also expressed in freshly
isolated cells (0 h) and did not decrease after 48h of culture
and further time points (P<0.01). These results also demonstrated
that expression of GRO-ƒ؟ by hepatocytes increased
in response to heat shock at different time points in comparison
with the control (P<0.01). These results demonstrated
that the isolation and heat shock stresses induced the
expression of GRO-ƒ؟ in hepatocytes in a time-dependent
manner. Thus, it seems that hepatocytes mimic the experiences
that the liver encounters after injury in vivo. In such a
situation, liver produces stress related agents like
chemokines to overcome injurious conditions.
