عنوان به زبان ديگر :
Expression and Purification of Homeodomain TGIFLYas a GST-Fusion Protein
پديد آورندگان :
Sarkargar F نويسنده , Ahani R نويسنده , Haji Hosseini R نويسنده , Raoofian R نويسنده , Youssefian L نويسنده , Heidari M نويسنده
چكيده لاتين :
Homcobox genes encode transcription factors which play important roles in the
developmental processes of many multicellular organisms, TGIFLX/Y (TG/FLX
and TG/FLY) are members of the homeobox superfamily of genes. Their
expressions are specifically detected in the human adult testis but their functions
are remained to be investigated. In this investigation we cloned full length of
TGIFLY eDNA and produced recombinant GST-TGIFLY protein in bacterial
system. Here we present production of GST-TGIFLY fusion protein as a soluble
protein. The recombinant protein was confirmed by western blot analysis using
anti-GST antibody. Through a single purification procedure using MagneGST
Beads, approximately 20 mg of the recombinant protein was obtained per liter of
bacterial culture. We suggest that GST-TG IFLY fusion protein could be utilized
as a valuable molecular tool on investigation of TGIFLY target genes and
identification of co-factors or partner proteins involved in TGIFLY function in
normal and abnormal development.
