20380

Determination of effective dosage of FSH and heG in the maturation of preantral follicles and enclosed oocytes in mice

JAVED AISHA نويسنده , Rezaei Zarchi Saeed نويسنده , Anvari Morteza نويسنده , Javeed Ghani Madiha نويسنده , Barzegari Firouzabadi Fatemeh نويسنده , JAMIL AMER نويسنده , Kalantar Seyed Mehdi نويسنده , Nazem Habibollah نويسنده

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Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM is an intellectual, scientific and clinical challenge with a number of potential applications. Objective: The effect of hCG was evaluated on the timing and regulation of in vitro ovulation for the Syrian mice oocytes in the presence and absence of FSH. Materials and Methods: Preantral follicles, isolated from the ovaries of 6 weeks-old mice, were cultured in TCM-199 medium. The effect of 10-200 mll.l/ml FSH and 1.5 IV/m) hCG was seen on the follicle maturation, as well as the changes in ovulation capacity of enclosed oocytes, after the incubation period of 6 days at 37°C, 92% humidity and 50/0 CO2 in air. Results: 100 mIV/ml FSH showed increased follicle diameter, survival, germinal vesicle breakdown (GVBD) and oocyte maturation rates (p