20592

Boophilus microplus Pyrethroid Resistance Associated to Increased Levels of Monooxygenase Enzymatic Activity in Field Isolated Mexican Ticks

Cossio-Bayugar Raquel نويسنده , Miranda-Miranda Estefan نويسنده , Ortiz-Najera Alejandrina نويسنده , Neri-Orantes Salvador نويسنده

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This study was made to assess SDS-PAGE monooxygenases zymograms in the presence of synthetic monooxygenase enzyme substrates, in order to establish a reliable methodology to measure monooxygenase activity levels on acaricide resistant ticks and finding a probable association between monooxygenase enzymatic levels and B. microplus acaricide resistance. Monooxygenase activity was found in the zymograms of the analyzed ticks at three polypeptidic bands of 147, 125 and 98 kDa, densitometric measurements of monooxygenases zymograms at these specific enzymatic bands showed a statistically significant monooxygenase activity increase (p