20593

Canola and Mustard Seeds/Sprouts Reduce the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colon Cancer in Fisher 344 Male Rats

Clisby A. نويسنده , Verghese M نويسنده , Cebert E نويسنده , Walker L.T نويسنده , Field R نويسنده , Shackelford L نويسنده , Boateng J نويسنده , Chawan C.B نويسنده

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The aim of the current study was to determine the efficacy of selected canola or mustard seeds (S) and canola sprouts (SP) cultivars (5 and 10% levels) on azoxymethane (AOM) induced Aberrant Crypt Foci (ACF) in Fisher 344 male rats. Following a one week period of acclimatization Fisher 344 male rats were randomly assigned to groups (n = 6). At seven and eight weeks of age rats received two successive sIc injections of AOM in saline at 16 mg kg" body weight. Rats were killed by CO2 asphyxiation at 17 weeks of age. The number ofACF in the proximal and distal colon in the treatment groups ranged from 6.33 (5% Pacific gold) to 15.55 (5% Jetton) and 21.33 (10% Pacific gold) to 52.0 (5°A. Idagold). Glutathione-S-transferase activity (umol mg-I ) was significantly (p<0.05) higher in the groups fed canola or mustard seeds compared to the control. Among the treatment groups the highest GST activity (umol mg") was observed in the rats fed 10% Pacific gold group (32.50). The results from this study show that canola or mustard sprouts and seeds reduced the incidence of AOM-induced ACF and may possibly prevent the incidence of colon cancer.

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