چكيده لاتين :
Basic research on the autoimmune disease multiple sclerosis has been performed
mainly on its animal model namely experimental allergic encephalomyelitis. There are
many different approaches established to get this model. Despite the existence of many
references in literature in this regard, we have been faced with many difficulties
generating the model suitable for studying different therapies. After a long time of
challenging to get a reliable and replicable method, we came up with the following major
points: First, the key element for getting a maximum number of sick animals at a defined
time is to consider the most appropriate animal body weight (19-20 gr). Even though the
age of immunized animals (6-8 week old) is highlighted in literature, we found out that
body weight is of a greater importance. Secondly, because the only available susceptible
mice strain in Iran is C57/BL6, the choice of peptide for immunization would be myelin
oligodendrocyte glycoprotein (35-55 sequence of this peptide 200 ىg/animal). Finally,
pertussis toxin which is a costly reagent plays a key role in stimulating the immune
response. Altogether, we recommend that considering the above mentioned tricks and
tracks, one would definitely be able to generate a chronic progressive type of model, for
basic research on therapies of multiple sclerosis.
