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Detennination of Harmine and Harmaline in Peganum harmala Seeds by High-Perfonnance Liquid Chromatography

Monsef-Esfahani Hamid Reza نويسنده , Faramarzi Mohammad Ali نويسنده , Mortezaee Venus نويسنده , Amini Mohsen نويسنده , Rouini Mohammad Reza نويسنده

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A High-Performance Liquid Chromatography (HPLC) mefhod was developed for determination of harmine, harmaline, harmol andhannalol in the extract of Peganum harmala seeds. The sample preparation was performed using liquid-liquid extraction. Chromatographic separation was achieved with a Tracer Excel 120 ODSA (150*4.6 mm) column, using a mixture of potassium phosphate buffer (10 mM, pH 7.0): acetonitrile (100:30; v/v) as mobile phase, in an isocratic mode at 1.5 mL min^-1. UV detection (lambda = 330 nm) was used. The calibration curves were linear (r^2>0.998) in the concentration range of 0.5-20 mo g mL^-1 for all analytes. The lower limit of quantification for all analytes was 0.5 mo g ml^-1. The within and between day precisions in the measurement of QC samples at three tested concentrations were in the range of 0.6-10.2% for all analytes. The HPLC method is able to measure the harmala alkaloids in the plant extract. The method has suitable reproducibility, sensitivity and resolution for routine and accurate use with UV detection

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