چكيده فارسي :
This study aimed to evaluate the benefits of using platelet– rich plasma (PRP) on wound healing. The study performed onten adult male dogs, aged 2-3 years and their weight were 18-25 kg. Under the effect of deep sedation and local subcutaneousanesthesia at the site of wound, four of (2x2) cm of full-thickness square cutaneous wounds were induced, two on each side ofthe lateral thoracic region of each animal. A pair of left wounds was treated by injection with 5 mL of autonomous PRP(treatment group), 2 mm lateral to the wound edges and in the wound center., immediately after wounding while, rightwounds, were injected with a single dose of 5ml of sterile saline (control group) by using the same procedure as in treatmentgroup. The clinical and histopathological evaluation of each group for five weeks, histological biopsies were taken in the days(3, 7, 14, 21, 28 and 35) of the experiment. The clinical evaluation of PRP treated wounds showed that the wound healingprocess (% contraction, % re-epithelization and % total wound healing) were significantly more (P 0.05) than the controlwounds during the five weeks study. The histopathological results revealed that PRP treated wounds have enhanced cellularity,increased vascularity, with increased amount of granulation tissue accompanied by increase numbers of skin appendagessuggesting enhanced cutaneous regeneration, than those in untreated wounds. Depending on the clinical and histopathologicalfindings, this study confirms that local implantation of PRP leads to acceleration and improvement cutaneous wound healing.
كليدواژه :
Dogs , Wound healing , Platelet , rich plasma , Growth factors , injery , skin
