تحضير احد مشتقات الكودائين كدواء مصاحب ودراسة تاثيره على بعض المتغيرات الكيموحيوية في امصال دم الارانب

Synthesis of codeine derivative as -a prod rug and study its effect on some biochemical parameters on rabbits

The codeine was used as carrier of alcohol to attach with non-steroidal anti-inflammatory Drug (aspirin ). The present work is aimed to synthesize the esterification reaction of (OH) group of codeine with aspirin as (acid chloride) which yielded the corresponding aspirin ester . The purity of the synthesized compounds were established by (TLC) and column chromatography , while the structures of their mutual prod rug was confirmed by (FTIR, 1HNMR,13CNMR) The results obtained give a good evidence for proposed structures to their compounds. The study included 30 rabbits with the same weights thy divided into three groups(ten rabbits for each group). First was a control group which did not have any dose, the second group has aspirin (0.099gm/kg) and codiene (0.07 gm/kg) dose ,and the third group contained the prepared derivative (0.159gm/kg)dose that equivalent with the dose of the second group. samples of blood have been takes from each rabbit, after three hours and serum has been separated to use in the study of the following parameter . The measured parameters include :the activity of (Lactate dehydrogenase(LDH) , Cholenestrase, creatinkinase (MB) ,Alkaline phosphatase(ALP) , alamine amino transaminase (ALT) , aspartae amino transaminase (AST) , cholinesterase and peroxidase) , and the concentrations of ( Cholestrol ,try clysride (T.G) , albumin and total protein ) and in addition to lipoproteins ( HDL , LDL, vLDL ) .and the concentration of total focus ,protein bounding focus and protein bounding hexsose. To ensure the release of pro drugs (Aspirin, codiene) a hydrolytic study of their esters were done at different pH [ 2 , 4 , 8 ,10 ] at constant temperature (25 C0) ,The results indicated that the hydrolysis at basic pH were faster than acidic pH which means that the most hydrolysis will be obtained at intestine and not in the stomach