Path Analysis منابع مقاومت آرابيدوپسيس به قارچ گندزي Botrytis. cinerea L با استفاده از دادههاي ميكرواري

Path analysis correlation of Botrytis fungi resistant gens in Arabidopsis with used of micro array data

به منظور آناليز بيان ژن هاي موثر بر مقاومت به قارچ Botrytis داده هاي ميكرواري مربوط به آزمايش inoculation قارچ Botrytis كه شامل 22800 پروب ژن بود، تهيه و با استاندارد كردن اين داده ها، ژن هايي كه co-over expression بسيار بالا داشتند، انتخاب شد و همبستگي پروب ها مربوط به منابع متفاوت (ژن هاي دفاعي) به دست آمد. سپس با استفاده از analysis Path2 اثرات مستقيم و غير مستقيم هر يك از ژن هاي موثر در استراتژي دفاعي گياه محاسبه شد. ژن هاي كه تاثير مستقيم روي استراتژي ژن هاي دفاعي داشتند، توالي پروموتوري آن ها مورد آناليز Sequence3 براي TF Binding Site4 قرار گرفت. نتيجه نشان داد وجود سايت هاي اتصال TF5 مشابه، از جمله SATB و Myb به طور معني دار (0.001>Pvalue) مهمترين عامل co-expression ژن هاي موردنظر بوده است. TF ها بعنوان عوامل مشترك باعث over expression بالا و هم بياني Network6 مجموعه ژني تحت تاثير قارچ Botrytis نشان مي دهد كه مي توان از نتايج آن در مهندسي ژنتيك جهت افزايش بيان مجموعه ژني براي ايجاد مقاومت با دستورزي ژنتيكي روي ژن فاكتور رونويسي TF موثر انجام داد.

In order to analyze effective genes on Botrytis fungi resistance, Botrytis fungi inoculation microarray data including 22800 gene probes, were prepared and the genes with high co-over expression were selected by standardization of these data. Then probes` correlation (probes which were related to different sources of defense genes) was obtained. Using Path Analysis, direct and indirect effects of each effective gene in Plant Defense Strategy (Anti fungal strategy) were calculated. The putative lectin similar lectin gene() has the most direct effect on anti fungal strategy. The promoters of genes with direct effect on P.D.S have been sequenced for TF binding site. Results showed that the existence of similar TF binding site such as SATB & Myb, were significantly the most important factors on studied genes co-expression (P<0.001). Translation binding site as a common factor causes high over expression and gene collection expression under effect of Botrytis fungi showed that its results can be used in genetic engineering for increment of gene collection expression in order to develop plant resistance by using genetic engineering on TF effective gene