بررسي تاثير نيكل و كينتين بر فعاليت آنزيم سوپراكسيد ديسموتاز دركالوس گياه بيش انباشتگر نيكل،Alyssum inflatum

The study of affecting kinetin and Ni on superoxide dismutase enzyme activity in Alyssum inflatum callus

گياه A. inflatum بومي خاك هاي سرپنتين غرب ايران بوده و بيش انباشتگر نيكل محسوب مي‌شود. در اين مطالعه از روش كشت كالوس استفاده شد تا با استفاده از سيستمي با درجات پيچيدگي كمتر از نظر سازمان بافتي و اندامي نسبت به گياه كامل، تاثير كينتين بر سوپراكسيد ديسموتاز (SOD, EC 1.15.1.1) به عنوان يك آنزيم آنتي اكسيداني درگير در مقاومت به فلزات بررسي شود.

Aim: A. inflatum is an endemic of serpentine soils from western Iran and hyperaccumulates Ni. In this study, we used culturing callus to evaluate the effect of kinetin on SOD (EC1.15.1.1) as an anti-oxidant enzyme engaged in resistance to metals, using a system less complicated than a developed plant both in texture and in organ. Material and methods: After reproducing the callus in MS medium with the concentration of 2,4-D at 1 mg/l and 0.1 mg/l for kinetin, the callus were kept under the treatment of (0, 200, 400 μM) Ni and (0, 0.1, 0.5, 1, 2.5 and 5 mg/l) kinetin and then the activity of antioxidant enzyme SOD was measured. Results: The activity of SOD enzyme in the absence of Ni and at concentrations of 1, 2.5 and 5 mg/l kinetin was increased. At 200 μM Ni concentration in the medium, none of kinetin concentrations showed an increasing effect on enzyme activity. At 400 μM concentration of Ni in the medium, two concentrations of 2.5 and 5 mg/l of kinetin had increasing effect on enzyme activity and as a result it provides resistance to Ni. Conclusion: Two concentrations of 2.5 and 5 mg/l of kinetin in the absence of Ni and also when Ni concentration is in the range of toxicity for callus, could be considered as an activity enhancer for the enzyme.