Synthesis and in-vitro evaluation of smart nanogel for cisplatin delivery

Cisplatin, cis-diamminedichloroplatinum(II) (CDDP), is a chemotherapy drug used in the treatment of solid tumours; but it showes significant dose-related sideeffects such as nephrotoxicity, neurotoxicity, nausea and vomiting. In order to maintain the efficacy and reduce the undesired disadvantages of cisplatin, Nanoparticle-based drug delivery systems were designed[1].Among the various drug nanocarriers , synthetic polymer nanoparticles have devoted more attention for reason of their excellent properties including biocompatibility and biodegradability particularly easy chemical modification or surface functionalization. A large number of these nanoparticles can respond to various stimulus such as temperature, magnetic, light, PH, enzymes, glucose and etc.These are termed as ‗smart‘or ‗intelligent‘. Introducing stimulus-responsive property to synthetic polymer nanoparticles can led to better therapeutic applications[2].In the present study, A dual-responsive nanogel, poly(N-isopropylacrylamide-co-N,N-(dimethylamino)ethyl methacrylate) P(NIPAAM-co-DMA) containing NIPAAM as thermoresponsive monomer and DMA as PH-responsive monomer and methylene-bis-acrylamide as cross linking agent, was synthesized by emulsion polymerization(Figure1). Cisplatin was loaded into the nanogel, by the swelling method. Properties of this nanogel such as size, swelling ratio, drug loading efficiency, and drug release kinetics were evaluated in vitro for controlled drug delivery in different temperature and PH conditions. Nanogelshowed significantly higher cisplatin release at 42°C compared to 37°C and at PH5.7 compared to PH7.4, demonstrating its temperature and PH sensitivity respectively.