Phytochemical Content of Aerial Parts of Salvia Limbata From Sabzevar-Iran

Salvia limbata is one species of the genus salvia which is belongs to the family of lamiaceae[1]. The wide variety of secondary metabolites such as flavonoids, tannins, and terpenoids were found in the essential oil of the plant that investigated for their pharmacological properties like analgesic; anti-inflammatory;hemostatic; antioxidant; antimicrobial and as an antitumor remedy[2]. Inthis study, we investigate the presence of some phytochemical compounds such as phenolic, flavonoid, alkaloid, anthraquinone, terpenoid, and steroids compounds in the extracts of aerial parts of S.limbata.The plant was collected from Sabzevar in May 2015. First of all, the plant part of S. limbata include flower and leaf were dried at room temperature, gritted, and steeped in methanol for 72 hours at room temperature. Then thecrude extract was condensed with rotary and fractionated with n-hexane,ethyl acetate and water, respectively. Finally different reagents were applied on TLC or extracts solution to screen of the presence of group of compounds. Wagner regent (I2+KI) for alkaloids(produce of brownish precipitate); H2SO4and CHCl3 for steroeids and terpenoids compounds (formation two phase); FeCl3 (1%) for phenolic compounds; KOH (5%) for anthraquinons, and AlCl3 (1%) for flavonoids( dark green, red, and yellow points on the TLC profile respectively)[3].The results are shown in Table 1.