Application of layered by layered polyaniline/graphene coating for solid phase microextraction of polycyclic aromatic hydrocarbons from food and environmental samples

A novel solid phase microextraction (SPME) method using the layered by layered Polyaniline/graphenized graphene coating made by electrochemistry methods were developed for determination of polycyclic aromatic hydrocarbons (PAHs) in food and environmental samples. Various coating methods were used for SPME: dipping technique, sol- gel technology, physical deposition and electrochemical modification [1]. Polycyclic aromatic hydrocarbons resulting from the incomplete combustion or pyrolysis of organic matter during industrial processing and various human activities [2]. These compounds are widely present in the environment due to their hydrophobic properties, allowing their adsorption onto atmospheric particles and direct deposition in sediments, soils and plants [3]. In this study a fiber coated with layered by layered Polyaniline/graphenized graphene made by electrochemistry methods were applied for extraction of (PAHs), as model analytes. These new fibers have large specific area, high chemical stability, good extraction efficiency and low cost. Six important parameters, affecting the extraction efficiency of PAHs, including: adsorption and desorption times, extraction solvent, pH, stirring rate and salt content of the sample were evaluated. Under experimental optimized conditions, the calibration curves are linear (R2≥0.996) in the concentration range of 0.01-300 µg/L, and the limit of detection(S/N=3) were under 0.001 µg/L for different PAHs. The applicability of the proposed method was evaluated by the extraction and determination of PAHs from several food and environmental samples.