Preconcentration and determination of acetaminophen using of sol-gel technique and hollow fibresupported by multi-walled carbon nanotubes functionalized magnetic nanoparticles

Acetaminophen is a popular active ingredient in the pharmaceutical industry and is widely used as an antipyretic medicament. acetaminophen does notcreate any harmful side effects, however, hypersensitivity or overdoses of acetaminophen lead to hepatotoxicity and nephrotoxicity and can also cause pancreatic diseases[1, 2].The sol–gel Technique provides a controlled condition to synthesize inorganic polymer and organic–inorganic hybrid materials. Sol–gel process can occur under extraordinary mild conditions, so it can be used to obtain products with various sizes, shapes and formats (e.g. fibers, films, monoliths and mono-sized particles) [3,4]. In this study, determination of trace amounts of acetaminophen in aqueous and urine samples is presented using hallow fiber solid phase microextraction method mediated functionalized multi-walled carbon nanotube.In this method, has been applied a silica-based organic-inorganic polymer that is attached to MWCNTs@Fe3O4. This polymer was prepared using the sol-gel method and the reaction between an acidic catalyst and tetra ortho silicate (TEOS). Sol was injected into the lumen ofhallow fiber that be converted to gel inits inside and this device has been used for direct extraction analyte sample. This method has advantages such as easy to preparation, wide application and high performance. Significant factors in extraction process contains pH of aqueous solution, adsorption and desorption time, volume of aqueous and organic phase, stirring rate and salt effect, were optimized using experimental design(CCD) by design expert software. Under the optimization condition, the linearity method was in the range of 0.0003-0.05 µg/mL with correlation coefficient (R2) 0.90. Limit of detection (LOD) and limit of quantification (LOQ) were obtained 3.705×10-4, 1.2×10-3, respectively. Finally, this method was applied for determination of this drug in the urine samples in three concentration 0.3, 0.0008, 0.0003 µg/mL for three replicate intraday with RSD=1.34 %. Pre-concentration factor (PF) is acquired 5692. 96.