Determination of hydrogen peroxide with using enzyme-free biosensor based on electrochemically reduced graphene oxide

The determination of hydrogen peroxide (H2O2) has played an important role in biomedical, environmental, and industrial analyses. During the past few decades, many enzyme based biosensors towards H2O2 determination have been made. However, many disadvantages were found, such as the high cost and instability of enzymes, critical demand on the environmental condition, and low reproducibility. Considering these facts, there has been more and more interest in enzyme-free H2O2 sensors, including electrodes modified with noble metals , metal alloys , metal oxides [1],carbon materials [2] and so on.Graphene as a new two-dimensional carbon material, has now captured the attention of scientists world wide due to its huge surface area, low cost, high electrical conductivity, and good mechanical and thermal properties [3]. For the fabrication of thin films of graphene on conducting substrates, direct electrochemical deposition is mostly used [4]. In this paper, we determination of hydrogen peroxide with enzyme-free biosensor (called non-enzymatic biosensor) using electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE). Graphene oxide (GO) was prepared from graphite powder using modified Hummer s method and drop cast on electrode surface. The ERGO was synthesized by electrochemically reducing GO and it was carried out using CV technique in a standard three electrode cell.