شماره ركورد كنفرانس :
3933
عنوان مقاله :
Quantitative determination of trace buprenorphine in human plasma by high performance liquid chromatography: Application of magnetic dispersive solid phase microextraction
پديدآورندگان :
Rostamkhani Shiva - Azarbaijan Shahid Madani University, Tabriz , Habibi Biuck - Azarbaijan Shahid Madani University, Tabriz , Hamidi Mehrdad hamidim@zums.ac.ir Zanjan Pharmaceutical Nanotechnology Research Center (ZPNRC), Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences;
عنوان كنفرانس :
بيست و چهارمين سمينار ملي شيمي تجزيه انجمن شيمي ايران
چكيده فارسي :
A novel, simple, available while reliable method was developed and validated for quantitation of buprenorphine (Bup) as a model highly lipophilic drug in human plasma. Bup a semi – synthetic opioid, has been widely used for treatment of chronic pain and for maintenance therapy for opioid addiction [1]. The quantitative determination of Bup concentration in human urine or plasma is problematic, thus necessitating sophisticated and expensive techniques like LC-MS and GC-MS [2-4]. The method is based on a rapid and easy dispersive solid phase microextraction using magnetic nanoparticles made of magnetite, (Fe3O4), core surrounded by an oleic acid shell, followed by high performance liquid chromatographic analysis with fluorescence detector. A series of process variables were tested for their possible effects on the adsorption/desorption of analyte or the HPLC-FL output. Bup could be quantitatively extracted using 4 mg MNPs and the whole extracted analyte could be easily desorbed with ethanol before injection to HPLC-FL. For the purpose of achiving a valid set of data Buprenorphine Ethanoate was synthesized and used as Internal Standard. A linear regression was found within the drug concentration range of 5to1000 ng/ml with R2 of 0.9998 for a typical calibration curve. The relative recovery of Bup was about 90% throughout the concentration range tested. Within-run and between-run variations were less than 10% within the linear range tested. Remarkably high sensitivity of 0.16 ng/ml and 0.55ng/ml was obtainedas limit of detection and a limit of quantification, respectively. This method, having excellence performance as well as validity is suggested strongly to be used in biomedical applications considering its ease of preparation and application while showing remarkable analysis validation data comparable to those reported for the sophisticated methods such as LC-MS or GC-MS.
