پديدآورندگان :
Milani-Hosseini Seyed Mohammad-Reza drmilani@iust.ac.ir Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, Tehran, Iran;: , Mohammadnezhad Parizad - Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, Tehran, Iran , Saeedzadeh Amiri Nasibeh - Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, Tehran, Iran
چكيده فارسي :
Graphene quantum dots(GQDs), as a recently discovered member of fluorescent nanomaterials, have gained tremendous interest for their unique optical performance [1,2]. Compared with the conventional nano opticalmaterials, GQDs exhibit the advantages of both admirable biocompatibility coupled to virtually non-existenttoxicity[3]. Molecularly imprinted polymer that can mimic the natural recognition entities has high affinity and selectivity for the specific analyte [4]. In this work, a molecularly imprinted polymer (MIP)-coated quantum dot (QD) nanocomposite was synthesized and utilized as a fluorescence probe for the highly sensitive and selective determination of biotin in biological samples. When biotin is added to the medium that has a MIP@GQDs composite, fluorescence quenching of this analyte is happened which is related to the concentration of the biotin. This fluorescence behavior is used for well testing biotin with the detection limit and linear range of µM. Beside, the prepared sensors could well against the interferences from various biomolecules and be successfully used for the assay of biotin in human biological samples, exhibiting excellent specificity. The resulting composites were verified by X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR). The morphology of GQDs-embedded SMIP which was characterized by TEM.
