Functionalized hallow fiber Solid phase micro-extraction approach for mebeverine quantification in biological and aquoes samples with florecence technique

New case for study of Solid-phase microextraction technique using a hollow fiber-supported sol-gel combined with multi-walled carbon nanotubes was employed for determination of Mebeverine in aqueous samples. In this technique, a silica-based, organic–inorganic polymer containing functionalized multi-walled carbon nanotubes (MWCNTs) was prepared with sol–gel technology via the reaction of tetraethylorthosil-icate (TEOS) with an acidic catalyst (HCl). A polypropylene hollow fiber segment was immerged into the vial which is containing sol for in situ gelation [1]. This device operated in direct immersion sampling mode. The experimental setup is simple and affordable and the device is disposable, so there is no risk of cross-contamination or carry-over. Parameters affecting extraction such as pH of the aqueous solution, ageing and extraction times, aqueous sample volume, agitation speed was optimized [2]. Linearity was observed over a range of 0.3–140 ng.mL-1, with an estimation coefficient ( r2) higher than 0.9938. The limit of detection (LOD) was 0.1 ng.mL-1 (n = 3), and repeatability (RSD% = 3.4) was from the analyte concentrations (20 ng.mL-1) with three replicates for each within a single day. Finally, a pre-concentration factor of 865.3 was obtained for Mebeverine.