Production of Non Specific Protein Foot and mouth disease vaccine with 6PD50 potency

The prophylactic use of vaccines against exotic viral infections in production animals is performed exclusively in regions where the disease concerned is endemic, like IRAN and Middle east region in such areas Foot and Mouth Disease infection pressure is very high , to assure optimal protection the most effective vaccines are used however in areas considered to be free from FMD And in which there is the possibility of only limited outbreaks the use of differentiation of infected from vaccinated animals (DIVA). Well produce and High potent (= 6PD50) vaccine is one of the useful factors for decrease economic losses. The potent vaccine can prevent the catastrophic losses of herd production and more resistance to new strain or FMD virus subtype and prevent the Becoming animal carrier. This investigation is a trial to produce the NSP free FMD vaccine with 6PD50 potency. NSP free vaccine differentiate the vaccinated from infected animals, that very important for country surveillance strategy. For this purpose after inactivation of FMD virus types( O2010 , A2005 , A2013 , Asia ) and concentrate by Ultra filter and Purify by column chromatography and formulated with aquos adjuvant (Aluyminium Hydroxide) –Saponine and Special Buffers. 20 naïve (unvaccinated) cattle were held in 4 groups 5 animals , for Challenge and detection of NSP antibody (3ABC-ELISA) with double dose infection at different time interval , and taken serum sample according the time table. 21 days after vaccination the challenge virus inject and in one week monitoring the PD50 was calculated. The potency of vaccine was about 7PD50 and Non of the serum had shown Non specific Antibody.