پديدآورندگان :
Bakhtiarian A bakhtiar@tums.ac.ir Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; , Nikoui V Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran , Ostadhadi S Experimental Medicine Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran , Abbasi Maleki S Department of Pharmacology, Urmia Branch, Islamic Azad University, Urmia, West Azarbaijan, Iran , Imran Khan M Department of Pharmacology, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran , Khanavi M Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran , Giorgi M Department of Veterinary Sciences, University of Pisa, San Piero a Grado, Pisa, Italy
كليدواژه :
Thymus kotschyanus , Analgesic , Formalin test , Tail flick test , Pain
چكيده فارسي :
Objectives: Considering the wide side effects of synthetic pain killers, use of herbal drugs in folk medicine as analgesic agents is to be increased. In present work we tried to uncover the pharmacological potential of ethanolic extracts by using various animal models and also to explore the potent antinociceptive effect of plant.Materials Methods:The ethanolic extract from the aerial part of Thymus kotschyanus after intraperitoneal administration at doses of 50, 100, and 200 mg/kg was evaluated against pain using formalin test and tail flick test.Statistical evaluation was carried out by one-way analysis of variance followed Student-Newman-Keuls for formalin test and two-way repeated measures analysis of variance followed Bonferroni for tail flick test. P≤0.05 was considered statistically significant.Results Conclusion: Different doses of compound of Thymus kotschyanus100 mg/kg (P≤0.05) and 200 mg/kg (P≤0.001) induced analgesia in the early and late phase of the formalin test. Maximum analgesia, in acute and late phases was experienced at the dose of 200 mg/kg while dose of 50 mg/kg was ineffective. The 200 mg/kg of extractincreased the antinociceptive activity in 30 (P≤0.01), 45, 60 and 75 (P≤0.05) minutes after injection followed by formalin and tail-flick test in comparison to control. The significant effect for doses 100 and 200 mg/kg after injection of Thymus kotschyanus was observed at 60 minutes.The results obtained in this study highlight that aerial part extract of Thymus kotschyanus possesses analgesic properties in both acute and chronic inflammatory pain.
