پديدآورندگان :
Zangeneh Akram akramzangeneh@yahoo.com Microbiology section, Pathobiology Basic sciences department, Veterinary faculty, Razi University, Kermanshah, Iran. , Goodarzi Nader Microbiology section, Pathobiology Basic sciences department, Veterinary faculty, Razi University, Kermanshah, Iran , Zangeneh Mohammadmahdi Microbiology section, Pathobiology Basic sciences department, Veterinary faculty, Razi University, Kermanshah, Iran
چكيده فارسي :
Objectives: The active compounds of Steviaare various steviol glycosides (mainly stevioside and rebaudioside), which have 250–300 times the sweetness of sugar. Stevia rebaudiana is cultivated and used to sweeten food elsewhere in East Asia. There is no study on antidiabetic effects extract of the Stevia bitter fraction in world. Therefore, the aim of this study was determination of antidiabetic activity of this fraction in world. Materials Methods: Animals were housed in plastic cages and were maintained under conventional laboratory conditions of temperature (20-25 C), with a regular 12-h light/12-h dark cycle throughout the study. They were fed standard pellet chow and were allowed water ad libitum. We use from 24 mice in four groups (Group 1: Diabetic control mice received STZ (50 mg/kg, i.p.) and served as the diabetic control. Group 2: Diabetic mice received glibenclamide (500 µg/kg, p.o.). Group 3: Diabetic mice received ethanol extracts of Steviabitter fractions (200 mg/kg, p.o.). Group 4: Diabetic mice received ethanol extracts of Stevia bitter fractions (400 mg/kg, p.o.). ). The effect of extract on serum biochemistry such as glucose levels, total protein, albumin, and alkaline phosphatise (ALP)were measured. Results Conclusion: The amount of glucose, total protein, albumin, and ALP in day 16 that were killedas follows respectively: Group 1: 236.25±8.77, 6.20±0.40, 1.12±0.17, 346.50±123.95. Group 2: 130.20±7.85,5.46±1.69, 0.94±0.26, 339.80±156.61. Group 3: 129.40±21.05, 6.58±0.22,1.28±0.25, 312.80±82.67. Group 4: 117.20±15.64, 6.66±0.32, 1.30±0.25, 306.00±92.10. As they are seen amount of glucose and ALP reduced in Group 3 and 4 to Group 1 and 2 and amount of total protein and albumin increased in Group 3 and 4 to Group 1 and 2. In conclusion, our biochemical evaluations suggest the strong antidiabetic potential of ethanol extracts of Steviabitter fractions. Its antidiabetic activity may be related to increased insulin secretion activity.
