پديدآورندگان :
Sadeghi Sefiddashti M Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Tahmasebian Ghahfarokhi N Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Nafar Sefiddashti M Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Ahmadi E eahmadihf@yahoo.com Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Hassanpour H Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran , Nazari H Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
كليدواژه :
wild celery , sperm motility , in vitro aging , oxidative stress , antioxidant
چكيده فارسي :
Objectives: It has been shown that in vitro sperm incubation lead to progressive reduction in sperm motility and its oocyte fertilizing capacity. One of the reasons that have been proposed for this phenomenon is the deleterious effects of excessive ROS produced during sperm incubation period. Spermatozoa are susceptible to oxidative stress due to the high membrane content of polyunsaturated fatty acid and the intracellular deficiency of antioxidant enzymes. Kelussia odoratissima Mozzaf (wild celery; mountain celery) is a medicinal herb and grows at high altitudes (more than 2000 m). This self-growing plant is exclusively found in Iran. Antioxidant potential of KelussiaodoratissimaMozzaf is comparable to α-tocopherol and butylatedhydroxytoluene. The aim of this study was to evaluate the effects of aqueous extract of Kelussia odoratissima Mozzaf on ovine epididymal sperm that incubated for 4 hours at 37˚C. Materials Methods: Testes of sexually mature rams were collected in local abattoir and transported to the laboratory, where epididymal sperms were expelled from caudal epididymis into HEPES buffered TCM199. Sperms were incubated at 37˚C in HEPES buffered TCM199 at concentration of 10 × 106 sperm/ml for 4 hours with or without 0.01% (w/v) wild celery aqueous extract. Motility of fresh sperms and incubated sperms were analyzed by computer-assisted sperm analysis system (CASA). Each treatment was replicated 5 times anddata of sperm motility parameters were analyzed by One-way ANOVA using IBM SPSS 20. Differences at a level of P 0.05 were considered significant. Results Conclusion: Incubation of sperms at 37˚C caused significant decrease in the sperm motility and progressive motility (56.2±3.26 and 47.4±5.6% respectively) compared with extract (72.3±2.94 and 67.2±3.38%) and fresh groups (78.2±1.77% and 73.7±1.78%). This study showed that aqueous extract of Kelussia odoratissima Mozzafcan prevent detrimental effects of in vitro incubation of ovine epididymal sperms.,
